English 
Dr. Thomas
Herrling
Messung von UV-induzierten freien Radikalen
in der menschlichen Haut
Privatinstitut Galenus GmbH, Berlin
UVA generated free radicals in human skin were measured
by ESR (Electron Spin Resonance) spectroscopy and imaging. The short life
time of the generated free radicals and reactive oxygen species like •OH-;
and L• demands the application of spin traps (nitroxide spin labels)
for scavenging and accumulating to get a sufficient signal to Noise ratio.
The ESR spectroscopy and imaging measurements have shown that the free radicals in skin are mainly generated by UVA. Using ESR measurements the protective effect of UVA-filters and antioxidants in the skin could be distinctly detected.
The used microwave frequencies of 9.5 GHz (λ=3 cm)
and 3 GHz (λ= 9.5 cm) enable an undisturbed investigation of the
whole skin up to the lower dermis in contrast to optical detection methods
with λ=350 – 600 nm.
The ESR is a valuable method for the evaluation of the UVA protection
capacity of sunscreen products.
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